What Does proteinase k molecular weight Mean?

What Does proteinase k molecular weight Mean?

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Proteinase K can hydrolyse various peptide bonds underneath various problems, even beneath ones that are as well stringent to render other proteases inactive. By way of example, Proteinase K continues to be an Lively wide range of temperatures; it may remain Energetic approximately 65°C and remain Energetic for your broad variety of pH amongst 7.

Being a serine protease, proteinase K catalyzes the hydrolysis of peptide bonds adjacent for the carboxylic group of aliphatic and aromatic amino acids.

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During the extraction of DNA (or nucleic acids generally speaking), several contaminating proteins are current. Proteinase K is Utilized in a lot of DNA extraction protocols to digest these contaminating proteins.

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Soon after proteinase K digestion of cellular lysates, it really is vital to execute phenol extractions to separate higher molecular weight DNA. The removal of proteinase K. Phenol extraction also enables section portioning lipids, proteins and DNA distinctly.

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Recombinant Proteinase K is often a nonspecific serine protease that is helpful for typical digestion of proteins.